normal human hepatic cell line lo2  (Keygen Biotech)

Journal: Frontiers in Immunology

Article Title: Elevated MPP6 expression correlates with an unfavorable prognosis, angiogenesis and immune evasion in hepatocellular carcinoma

doi: 10.3389/fimmu.2023.1173848

Figure Lengend Snippet: MPP6 expression in HCC. (A) MPP6 mRNA expression in malignant tumor and normal tissues based on the TCGA database. (B, C) MPP6 mRNA expression in normal liver and HCC tissues based on GSE112791 (GPL570 platform) and GSE101685 datasets. (D) MPP6 mRNA expression in HCC cell lines (Huh7, Hep3B, BEL-7404, and SMMC-7721) compared with the normal hepatic cell line LO2 by qRT−PCR. (E) MPP6 protein expression in normal liver and HCC tissues based on the UALCAN website. (F) MPP6 protein expression in LO2 and HCC cell lines (Huh7, Hep3B, BEL-7404, and SMMC-7721) by Western blotting. (G) Immunohistochemical staining analysis of MPP6 in normal liver and HCC tissues. * P < 0.05; ** P < 0.01; *** P < 0.001; ns: P > 0.05.

Article Snippet: The human normal hepatic cell line LO2 was provided by Nanjing KeyGen Biotech.

Techniques: Expressing, Quantitative RT-PCR, Western Blot, Immunohistochemical staining, Staining

Journal: OncoTargets and therapy

Article Title: lncRNA TMPO-AS1 Exerts Oncogenic Roles in HCC Through Regulating miR-320a/SERBP1 Axis

doi: 10.2147/OTT.S250355

Figure Lengend Snippet: High TMPO-AS1 expression is identified in HCC cells and tissues. ( A ) The expression levels of TMPO-AS1 in HCC tissues and non-cancerous tissues were detected using qRT-PCR assay. ** P <0.01 compared with non-cancerous. ( B ) Expression levels of TMPO-AS1 in HCC tissues from stage I–II and stage III–IV. * P <0.01 compared with I–II. ( C ) Expression levels of TMPO-AS1 in HCC patients with metastasis and without metastasis. * P <0.01 compared with no metastasis. ( D ) Kaplan–Meier curves for HCC patients with higher expression of TMPO-AS1 or lower expression of TMPO-AS1. ( E ) The levels of TMPO-AS1 in HCC cell lines and LO2 cell were detected using qRT-PCR assay. ** P <0.01 compared with LO2. ( F ) Expression of TMPO-AS1 in control tissues (n=50) and liver hepatocellular carcinoma (LIHC) tissues (n=374). TMPO-AS1 expression is significantly upregulated in LIHC tissues compared with control tissues based on the analysis of the high-throughput sequencing database of TCGA.

Article Snippet: HCC cells (HepG2, SNU-387, HCCLM3, SMMC-7721, Huh7) and normal human hepatic cell line, LO2 were purchased from Jiangsu KeyGEN BioTECH (Nanjing, Jiangsu, China) and were maintained in RPMI-1640 or DMEM containing 10% FBS at 37°C in a 5% CO 2 incubator.

Techniques: Expressing, Quantitative RT-PCR, Control, Next-Generation Sequencing

Journal: OncoTargets and therapy

Article Title: lncRNA TMPO-AS1 Exerts Oncogenic Roles in HCC Through Regulating miR-320a/SERBP1 Axis

doi: 10.2147/OTT.S250355

Figure Lengend Snippet: TMPO-AS1 serves as a sponge of miR-320a. ( A ) The localization of TMPO-AS1 in cells (SNU-387 and HCCLM3) was confirmed by nuclear-cytoplasmic fractionation. ( B ) The potential binding sites between miR-320a and TMPO-AS1 were hypothesized using bioinformatics analysis starBase v.2.0 database. ( C ) The targeted binding effects between miR-320a and TMPO-AS1-wt or TMPO-AS1-mut in SNU-387 and HCCLM3 cells were detected using luciferase reporter assay. ** P <0.01 compared with miR-NC. ( D ) RIP assay was conducted to confirm the binding ability between TMPO-AS1 and miR-320a. Both TMPO-AS1 and miR-320a were significantly enriched in Ago2 immunoprecipitate. ** P <0.01 compared with anti-IgG. ( E ) RNA-FISH analysis showed that miR-320a co-localizes with TMPO-AS1 in SNU-387 and HCCLM3 cells. ( F ) The relative expression of miR-320a in SNU-387 and HCCLM3 cell transfected with sh-TMPO-AS1 or pc-TMPO-AS1 was measured by RT-qPCR. ** P <0.01 compared with sh-NC. ( G ) The levels of miR-320a in HCC cells and LO2 cells were determined by qRT-PCR assay. ** P <0.01 compared with LO2. ( H ) Cell viability of SNU-387 and HCCLM3 cell after miR-320a upregulation was conducted by performing CCK-8 assay. ( I ) The proliferation of miR-320a overexpressed SNU-387 and HCCLM3 cells was examined by colony formation assay. ( J ) The migration of miR-320a overexpressed SNU-387 and HCCLM3 cells was examined by wound healing assay. ( K ) The invasion of miR-320a overexpressed SNU-387 and HCCLM3 cell was examined by Transwell invasion assay. ** P <0.01 compared with control.

Article Snippet: HCC cells (HepG2, SNU-387, HCCLM3, SMMC-7721, Huh7) and normal human hepatic cell line, LO2 were purchased from Jiangsu KeyGEN BioTECH (Nanjing, Jiangsu, China) and were maintained in RPMI-1640 or DMEM containing 10% FBS at 37°C in a 5% CO 2 incubator.

Techniques: Fractionation, Binding Assay, Luciferase, Reporter Assay, Expressing, Transfection, Quantitative RT-PCR, CCK-8 Assay, Colony Assay, Migration, Wound Healing Assay, Transwell Invasion Assay, Control